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surface cd33 ac104 3 e3 142 miltenyi 4 3 surface cd11c  (Miltenyi Biotec)


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    Miltenyi Biotec surface cd33 ac104 3 e3 142 miltenyi 4 3 surface cd11c
    Surface Cd33 Ac104 3 E3 142 Miltenyi 4 3 Surface Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd33+car+detection+reagent/CD33+CAR+Detection+Reagent%2C+human/pmc12477255__Supplementaryfile1-19-40-44
    Average 93 stars, based on 3 article reviews
    surface cd33 ac104 3 e3 142 miltenyi 4 3 surface cd11c - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Primary CD33-targeting CAR-NK cells for the treatment of acute myeloid leukemia
    Article Snippet: GFP-positive tumor cells or CD56 and CD33-CAR expression were analyzed using a BD FACSCelesta flow cytometer. .. An anti-CD56-BV786 antibody (Clone NCAM16.2, BD Biosciences) and a CD33-CAR Detection Reagent (containing a recombinantly expressed fusion protein consisting of the human CD33 extracellular domains and a specifically mutated human IgG1 Fc region, Miltenyi Biotec) followed by an anti-biotin-PE antibody (clone REA746, Miltenyi Biotec) were used to determine CD56 and CD33-CAR expression, respectively. .. Cytokine release of CAR-NK and UTD-NK cells co-cultivated with AML cell lines or primary AML cells as well as cytokine levels of blood from mice three days pre tumor cell injection and one day post therapy initiation (four days post tumor cell application) were determined using a BD Cytometric Bead Array (CBA; BD Biosciences) or MACSPlex Cytokine Assays (Miltenyi Biotec).

    Staining:

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33-protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag from a human Single Cell Sample Multiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells.
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag froma humanSingle Cell SampleMultiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.

    Labeling:

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33-protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag from a human Single Cell Sample Multiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells.
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag froma humanSingle Cell SampleMultiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.

    Single Cell:

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33-protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag from a human Single Cell Sample Multiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.

    Multiplexing:

    Article Title: CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells
    Article Snippet: .. Subsequently, cells were stained with an anti-NKG2A-PE antibody (Clone Z199, Beckman Coulter), CD33-CAR-detection reagent (CD33-protein-biotin + anti-biotin-APC (Clone RE746, Miltenyi Biotec) depending on the cell type and each condition was labeled with a different SampleTag from a human Single Cell Sample Multiplexing Kit (BD Biosciences). .. Subsequently, cells were stained with 7-AAD (BD Biosciences) to exclude dead cells and were sorted using a BD FACSAria machine.



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    a Scheme of the experimental setting to compare SS-CAR-NK and PROD-CAR-NK cells in vivo. b Scheme of the second-generation CAR-construct, which was incorporated into the NK cells. CAR-expression ( c ), NK cell expansion ( d ) as well as NK cell purity ( e ) and CD3 + T cell contamination ( f ) at day 14. g Flow-cytometry analysis of cell surface expression of different cell surface markers on SS-CAR-NK and PROD-CAR-NK cells. h SS-CAR-NK and PROD-CAR-NK cell in vitro cytotoxicity assay against <t>CD33</t> + OCI-AML2 cells at low effector-to-target-ratio. Cells were co-cultivated for 4 h and dead AML cells quantified as DAPI + by flow cytometry. Shown is one experiment with technical triplicates. Mean ± SD. i Scheme of the in vivo comparison of SS-CAR-NK and PROD-CAR-NK cells in an OCI-AML2 NSG-SGM3 xenograft mouse model. Mice received a single dose of 1 ×10 7 NK cells at day 3 post AML cell injection followed by subcutaneous treatment with IL-2. j Total flux analysis (photons/second) as well as bioluminescence images (BLI) ( k ) of differently treated OCI-AML2 (luciferase + ) NSG-SGM3 xenografted mice over time after subcutaneous injection of luciferin ( n = 6–9 mice/per group).
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    a Scheme of the experimental setting to compare SS-CAR-NK and PROD-CAR-NK cells in vivo. b Scheme of the second-generation CAR-construct, which was incorporated into the NK cells. CAR-expression ( c ), NK cell expansion ( d ) as well as NK cell purity ( e ) and CD3 + T cell contamination ( f ) at day 14. g Flow-cytometry analysis of cell surface expression of different cell surface markers on SS-CAR-NK and PROD-CAR-NK cells. h SS-CAR-NK and PROD-CAR-NK cell in vitro cytotoxicity assay against <t>CD33</t> + OCI-AML2 cells at low effector-to-target-ratio. Cells were co-cultivated for 4 h and dead AML cells quantified as DAPI + by flow cytometry. Shown is one experiment with technical triplicates. Mean ± SD. i Scheme of the in vivo comparison of SS-CAR-NK and PROD-CAR-NK cells in an OCI-AML2 NSG-SGM3 xenograft mouse model. Mice received a single dose of 1 ×10 7 NK cells at day 3 post AML cell injection followed by subcutaneous treatment with IL-2. j Total flux analysis (photons/second) as well as bioluminescence images (BLI) ( k ) of differently treated OCI-AML2 (luciferase + ) NSG-SGM3 xenografted mice over time after subcutaneous injection of luciferin ( n = 6–9 mice/per group).
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    Image Search Results


    a Scheme of the experimental setting to compare SS-CAR-NK and PROD-CAR-NK cells in vivo. b Scheme of the second-generation CAR-construct, which was incorporated into the NK cells. CAR-expression ( c ), NK cell expansion ( d ) as well as NK cell purity ( e ) and CD3 + T cell contamination ( f ) at day 14. g Flow-cytometry analysis of cell surface expression of different cell surface markers on SS-CAR-NK and PROD-CAR-NK cells. h SS-CAR-NK and PROD-CAR-NK cell in vitro cytotoxicity assay against CD33 + OCI-AML2 cells at low effector-to-target-ratio. Cells were co-cultivated for 4 h and dead AML cells quantified as DAPI + by flow cytometry. Shown is one experiment with technical triplicates. Mean ± SD. i Scheme of the in vivo comparison of SS-CAR-NK and PROD-CAR-NK cells in an OCI-AML2 NSG-SGM3 xenograft mouse model. Mice received a single dose of 1 ×10 7 NK cells at day 3 post AML cell injection followed by subcutaneous treatment with IL-2. j Total flux analysis (photons/second) as well as bioluminescence images (BLI) ( k ) of differently treated OCI-AML2 (luciferase + ) NSG-SGM3 xenografted mice over time after subcutaneous injection of luciferin ( n = 6–9 mice/per group).

    Journal: Bone Marrow Transplantation

    Article Title: Manufacturing of primary CAR-NK cells in an automated system for the treatment of acute myeloid leukemia

    doi: 10.1038/s41409-023-02180-4

    Figure Lengend Snippet: a Scheme of the experimental setting to compare SS-CAR-NK and PROD-CAR-NK cells in vivo. b Scheme of the second-generation CAR-construct, which was incorporated into the NK cells. CAR-expression ( c ), NK cell expansion ( d ) as well as NK cell purity ( e ) and CD3 + T cell contamination ( f ) at day 14. g Flow-cytometry analysis of cell surface expression of different cell surface markers on SS-CAR-NK and PROD-CAR-NK cells. h SS-CAR-NK and PROD-CAR-NK cell in vitro cytotoxicity assay against CD33 + OCI-AML2 cells at low effector-to-target-ratio. Cells were co-cultivated for 4 h and dead AML cells quantified as DAPI + by flow cytometry. Shown is one experiment with technical triplicates. Mean ± SD. i Scheme of the in vivo comparison of SS-CAR-NK and PROD-CAR-NK cells in an OCI-AML2 NSG-SGM3 xenograft mouse model. Mice received a single dose of 1 ×10 7 NK cells at day 3 post AML cell injection followed by subcutaneous treatment with IL-2. j Total flux analysis (photons/second) as well as bioluminescence images (BLI) ( k ) of differently treated OCI-AML2 (luciferase + ) NSG-SGM3 xenografted mice over time after subcutaneous injection of luciferin ( n = 6–9 mice/per group).

    Article Snippet: CAR expression was analyzed by using a CD33-CAR Detection Reagent which contained a recombinantly expressed fusion protein that consisted of the extracellular domains of human CD33 and a specifically mutated human IgG1-Fc region connected to biotin, and secondary a biotin-targeting antibody, anti-biotin PE (clone REA746) (both Miltenyi Biotec).

    Techniques: In Vivo, Construct, Expressing, Flow Cytometry, In Vitro, Cytotoxicity Assay, Comparison, Injection, Luciferase